Significant pairs of canonical variates indicated that poor physical activity and weight control (−0.77), poor dietary habits (−0.78), alcohol consumption and cigarette smoking (−0.37), lack of sleep and rest (−0.40), stress (−0.64) in the lifestyle set, higher negative affectivity (0.52), and social inhibition (0.71) in the type-D personality set were associated with a high MetS score (0.59) and severity of CAD (0.91). The behavioral-psychological variable set was related to the predictor set for CAD prognosis, with a significant canonical variate of 0.67 (45% overlapping variance). Descriptive analyses, t-tests, analysis of variance, Pearson’s correlation, and canonical correlation were used.
The behavioral-psychological variable set contained six lifestyle categories and two type-D personality categories. In this cross-sectional descriptive study, we aimed to investigate the relationship between behavioral-psychological variables and predictors of CAD prognosis. Unhealthy lifestyles and type-D personality are associated with MetS and are potential causes of primary and secondary CAD. The proto-oncogene c-MYC showed statistically significant upregulation in cancer tissue samples suggesting that the OSCC among South Indian population is primarily not mediated by the canonical Wnt signaling pathway.Ĭanonical Wnt pathway gene analysis oral squamous cell carcinoma quantitative reverse transcription-polymerase chain reaction.Metabolic syndrome (MetS) and severity of coronary artery disease (CAD) are considered predictors of CAD prognosis. However, in this study, there was no significant expression of all the three genes in OSCC. Wnt3A, β-catenin, and cyclin D1 are recognized as key components of Wnt/β-catenin signaling. Significant expression of Wnt3A was observed among patients who had recurrence and were deceased. All the analyses were carried out using SPSS version 23.0 (IBM SPSS Inc., USA).Įxpression of sFRP-1, sFRP-2, and sFRP-5 in control samples and expression of c-MYC and cyclin D1 in cancer samples showed statistical significance. If the data were normal, then parametric tests were used otherwise, nonparametric alternatives were used. Wnt-3a, β-catenin, secreted frizzled-related proteins sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wnt inhibitory factor 1, dickkopf-1, c-MYC, and cyclin-D1 from cancer (n = 29) and normal (n = 29) tissue samples were investigated using quantitative reverse transcription-polymerase chain reaction.ĭescriptive statistics were used to summarize the sample characteristics and clinical variables. Informed consent from patients and institutional ethical approval for the study was obtained and the guidelines were strictly followed for collection of samples.Ĭlinical data and mRNA expression analysis of ten genes in the canonical Wnt pathway were evaluated and their relationships with clinical and demographic variables were studied in 58 tissue samples.
The aim of this study is to explore the prognostic significance and clinicopathological correlations of the Wnt pathway genes in a cohort of surgically treated patients with oral squamous cell carcinoma (OSCC) patients.Ī prospective genetic study on patients with OSCC was carried out during the period from July 2014 to January 2016.